ABSTRACT
Objective To research and evaluate cell counting random error for cell sheet of T lymphocyte subsets in the practical McAb‐A‐E direct method ,and old method of McAb‐A‐E direct method compared .Methods Randomly selected 20 specimens ,cell sheet of T lymphocyte subsets on counting 200 cell at least 2 area from 2/6~4/6 area ,counting area non‐repeated ,CD4+ and CD8+continuous counter 4 times ,CD3+ continuous counter 2 times .Results In the practical McAb‐A‐E direct method ,random error mean of cell counting was separately CD3+ :7 .86 ,CD4+ :9 .99 ,CD8+ :8 .55 ,CD4+ /CD8+ :0 .33 ,lower than old method of McAb‐A‐E direct method ,that was CD3+ :15 .00 ,CD4+ :13 .80 ,CD8+ :10 .70 ,CD4+ /CD8+ :0 .69 .Conclusion Cell counting random error in practical McAb‐A‐E direct method for cell sheet of T lymphocyte subsets is less than old method of McAb‐A‐E direct method .